|
|
 |
Retinol Binding Protein EIA | | | General Information | |
| Vendor | | ALPCO Diagnostics | | Item | | Retinol Binding Protein EIA | | Catalog Number | | 030-6110 | | Size | | 96 Wells | | Range | | 5.5-165 µg/ml | | Sample Type | | Serum, Plasma, Urine | | Regulatory Status | | For Research Use Only | | Discipline | | Vitamin Assays / Nutrition / Miscellaneous, Bone and Mineral Assays | | Sensitivity | | 0.9 µg/l | | Incubation Time | | 2 hours and 20 minutes | | Pricing |  | Inquire |
| | Product Description | |
| Retinol binding Proetin (RBP) is small (21kD) transport protein for vitamin A
which forms a complex with pre-albumin in blood but loses its affinity for
prealbumin once the vitamin has been deliverd to the target cells. The free
RBP molecule is then rapidly filtered at the glomerulus and catabolized in the
renal tubules after resorption by the proximal tubular cells (like other small
molecules e.g. ß-2 Microglobulin). In kidney disease with prevailing tubular
changes these proteins are not reabsorbed and appear in the urine.
This Enzyme-Linked Immunosorbent Assay (ELISA) serves the quantitative
determination of the Retinol binding protein (RBP) from plasma, serum and
urine. In a 1 hour incubation step, the RBP in the samples is bound to an
available excess of polyclonal rabbit antibodies against RBP, which is
immobilized to the surface of the microtitre plates. After a washing step, to
remove all foreign substances, the quantification of bound RBP is carried
out by adding an enzyme labeled antibody, which also binds to the RBP.
The amount of converted substrate is directly proportional to the amount
of bound RBP and can be determined photometrically at 450 nm (if
extinction is out of range, measure at 410 nm). | |
|
 |
| ALPCO Diagnostics | Call ALPCO Diagnostics to buy products (US and Canada only; other locations use number below)   1-866-466-7731 |
| | Customer Service: (800) 592-5726 | | Web site: http://www.alpco.com | | Please mention Medcompare.com when you contact the vendor. |
| NOTE: Medcompare disclaims any information on this site. The information found on Medcompare (Drug Guide, Product Listings and otherwise) is intended for use by medical professionals. This information is not intended to replace the information, advice or instructions from a medical doctor. Pricing information is approximate, actual prices may vary. |
|
|
