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Insulin EIA | | | General Information | |
| Vendor | | ALPCO Diagnostics | | Item | | Insulin EIA | | Catalog Number | | 80-INSHU-E01 | | Size | | 96 Wells | | Range | | 3 - 200 uIU/ml | | Sample Type | | Serum, Plasma | | Regulatory Status | | For In-Vitro Diagnostic Use | | Discipline | | Diabetes and Obesity | | Sensitivity | | 0.798 ulU/ml | | Incubation Time | | 2 hours | | Pricing |  | Inquire |
| | Related Content | |
| | Product Description | |
| The ALPCO Insulin EIA is a sandwich type immunoassay. The 96 well microplate is coated with a monoclonal antibody specific for insulin. The Insulin Standards, Diabetes Antigen Controls and samples are added to the microplate wells with a horseradish peroxidase enzyme labeled monoclonal antibody. The microplate is then incubated on an orbital microplate shaker at 700-900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. Substrate is added and the microplate is again incubated on an orbital microplate shaker (700-900 rpm). Once the second incubation period is complete, Stop Solution is added and the optical density (OD) is measured by spectrophotometer at 450nm with a reference wavelength of 620nm. The intensity of the color generated is directly proportional to the amount of insulin in the sample. | |
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| ALPCO Diagnostics | Call ALPCO Diagnostics to buy products (US and Canada only; other locations use number below)   1-866-466-7731 |
| | Customer Service: (800) 592-5726 | | Web site: http://www.alpco.com | | Please mention Medcompare.com when you contact the vendor. |
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