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	Genetic Analysis Systems in the Clinical Mainstream

> > > C-Peptide EIA

C-Peptide EIA

General Information

Vendor		ALPCO Diagnostics
Item		C-Peptide EIA
Catalog Number		80-CPTHU-E01
Size		96 Wells
Range		20-3000 pM
Sample Type		Serum, Plasma
Regulatory Status		For In-Vitro Diagnostic Use
Discipline		Diabetes and Obesity
Sensitivity		2.95 pM
Incubation Time		2 hours
Pricing		Inquire

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Product Description

The ALPCO C-Peptide EIA is a sandwich type immunoassay. The 96 well microplate is coated with a monoclonal antibody specific for C-Peptide. The C-Peptide Standards, Diabetes Antigen Controls and samples are added to the microplate wells with Assay Buffer on an orbital microplate shaker at 700-900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. The horseradish peroxidase enzyme-labeled monoclonal antibody is then added, and the microplate is incubated a second time on an orbital shaker at 700-900 rpm, washed and blotted dry. Substrate is added, and the microplate is incubated a third time on an orbital microplate shaker at 700-900 rpm. Once the incubation period is complete, Stop Solution is added, and the optical density (OD) is measured by spectrophotometer at 450nm with a reference wavelength of 620nm. The intensity of the color generated is directly proportional to the amount of C-Peptide in the sample.

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