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Free Estriol ELISA |  | | General Information |  |
| Vendor |  | ALPCO Diagnostics | | Item |  | Free Estriol ELISA | | Catalog Number |  | 20-DR-1612 | | Size |  | 96 Wells | | Range |  | 0.3 - 40 ng/ml | | Sample Type |  | Serum | | Regulatory Status |  | For In-Vitro Diagnostic Use | | Discipline |  | Reproductive Hormones | | Sensitivity |  | 0.02 ng/ml | | Incubation Time |  | 1 hr and 30 min | | Pricing |  | Inquire |
 | | Product Description |  |
| The Estriol (unconjugated) enzyme immunoassay (EIA) test kit is to be used for the in vitro
determination of unconjugated estriol in human serum during the second half of pregnancy. The Estriol (unconjugated) procedure is an enzyme immunoassay, which is based on the
principle of competitive binding. In this procedure, estriol is conjugated to the enzyme
horseradish peroxidase. Serum containing estriol (i.e. standard or patient) is mixed with estriol
HRP conjugate and added to the wells on a microtiter strip. Estriol antibody is already precoated
in the microwells. After one hour, the free estriol horseradish peroxidase and unbound estriol
are washed from the wells with buffer. Substrate is added to the antibody bound estriol
horseradish peroxidase which is immobilized in the wells. After a brief incubation period a
stopping reagent is added, which gives rise to a colored product which is measured
spectrophotometrically utilizing either a microplate or microstrip reader. |  |
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| ALPCO Diagnostics | Call ALPCO Diagnostics to buy products (US and Canada only; other locations use number below) 1-866-466-7731 |
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